Xcelom Limited (Global)
Long-read sequencing
AltruType™
Long-Read Sequencing HLA Genotyping
Break the Ambiguity. Resolve with Confidence.
HLA Tpying Ambiguity
The Human Leukocyte Antigen (HLA) system is encoded by a family of genes on chromosome 6 and represents one of the most genetically variable regions in the human genome. It plays a central role in cellular immunity against foreign substances by encoding cell surface proteins that distinguish self from non-self. Given the critical importance of HLA molecules in immune responses, HLA typing is widely applied for different fields.
Currently, HLA typing is performed using a variety of techniques. However, even with high‑resolution NGS, commercially available assays still yield ~3% ambiguous results [1] because of low mappability in certain regions, phasing ambiguities, etc.
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Break the Ambiguity with LRS
AltruType™ offers a complete sample-to-report HLA typing workflow.
It delivers high-resolution results (up to 8-digit) with gold-standard level accuracy and unambiguous calls from high-quality sequencing data. Optimized for ease of use and cost-efficiency, AltruType™ is ideal for service laboratories.
To Dec 2025, >20,000 AltruType™ long-read HLA genotyping had completed and identified more than 140+ novel alleles.
Proprietary library preparation
Balanced, stable amplification system
AltruType™ adopts proprietary single-tube LR‑PCR enrichment to provide a stable, efficient foundation for downstream analysis.
Long‑range amplification reduces amplification imbalance, and long-read length span entire genes to minimize phasing ambiguity from alignment errors.
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Ultra-long amplicon (several kb) covers the most region
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Avoids allelic imbalance
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No fragmentation – fully phased alleles
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Proprietary software - TransMatch™
All-in-one for bioinformatics and reporting
TransMatch™ is a software for HLA sample management and results reporting, delivering end-to-end analytics and reporting for the service laboratory. Powered by dual‑algorithms cross‑validation, automated novel allele detection, and one‑click reporting function, it achieves exceptional HLA typing accuracy with an efficient workflow.
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Fully automated analysis, user-friendly
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Dual-algorithm – no ambiguous final call
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Regular database updates for novel & null allele
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Flagging candidates for novel allele
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Accurate haplotype phasing across complex region
Easy to operate at scale
Streamlined experimental workflow
Library preparation automation-ready
Short hands-on time
User-friendly software supports routine workflow
Automated analysis & reporting, sample management
No manual intervention at critical steps
Stable, reproducible results with LRS and proprietary pipline
Technology Transfer
Xcelom Limited and Haorui Genomics provide a comprehensive end-to-end technology transfer solution for conducting HLA typing tests within your facility.
From Sample to Report:
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Laboratory setup and consultation
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End-to-end Workflow: SOPs, reagent, and training
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Analysis Software: From sample management to report generation
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Platform
PacBio Sequel II, Vega and Revio system
Sample Type
gDNA from blood
Test per Batch
Up to 192 tests per SMRT cell
Operation Time
~ 40.5 hours (Hand on time: ~85 minutes)
Automation Capability
Yes
Coverage
Basic for 11 HLA loci - Class I & II: HLA-A, HLA-B, HLA-C, HLA-DRB1, HLA-DRB3/4/5, HLA-DQA1, HLA-DQB1, HLA-DPA1, and HLA-DPB1
Extended coverage: HLA-E, HLA-F, and HLA-G
Further Scope Expansion
In addition to our standard HLA typing solution, we offer expanded LRS-based immunogenetics typing panels. These solutions help service laboratories to broaden their offerings using the similar workflow, same sequencing run and analysis platform.
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ABO+H - Full-length ABO, FUT1, and FUT2 genes
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Rh - Full-length RHD, RHCE, and RHAG genes
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RHD - Full-length RHD gene
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RHCE - Full-length RHCE gene
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PANEL2 (ABO, Rh, MNS, Kidd, Duffy) - Full-length or key regions of ABO, RHD, RHCE, GYPA, GYPB, SLC14A1, and ACKR1 genes
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PANEL3 (ABO, Rh, MNS, Kidd, Duffy, P1PK, Kell, Lewis, Diego) - Full-length or key regions of ABO, RHD, RHCE, GYPA, GYPB, SLC14A1, ACKR1, A4GALT, KEL, FUT2, FUT3, and SLC4A1 genes
References:
1. Weimer ET, Montgomery M, Petraroia R, Crawford J, Schmitz JL. Performance Characteristics and Validation of Next-Generation Sequencing for Human Leucocyte Antigen Typing. J Mol Diagn. 2016;18(5):668-675.