Xcelom Limited (Global)
Long-read sequencing
AltruType™
Long-Read Sequencing HLA Genotyping
High-Resolution (8-Digit) HLA Typing without Ambiguity
HLA Typing Ambiguity
The Human Leukocyte Antigen (HLA) system is the most genetically variable region in the human genome. Accurate HLA typing is critical for transplant compatibility and disease association studies. Current Short-read NGS techniques often struggle with the complexity of the HLA region. Even high-resolution commercial assays can yield ~3% ambiguous results¹ due to phasing issues and low mappability in certain regions, etc.
Break the Ambiguity. Resolve with Confidence.
AltruType™ offers a complete sample-to-report HLA typing workflow.
It delivers high-resolution results (up to 8-digit) with gold-standard level accuracy and unambiguous calls from high-quality sequencing data. Optimized for ease of use and cost-efficiency, AltruType™ is ideal for service laboratories.
To Dec 2025, >20,000 AltruType™ long-read HLA genotyping had completed and identified more than 100+ novel alleles.
Proprietary Single-Tube Library Preparation
Balanced, stable amplification system
AltruType™ adopts proprietary single-tube LR‑PCR amplification to reduce amplification imbalance. The long-range amplicon covering the entire genes can minimize phasing ambiguity from alignment errors.
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Pre-mixed reagents & automation-ready protocols
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Avoids allelic imbalance - uniform coverage & distinguishes true homozygosity from potential allelic dropout
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No fragmentation – generates intact, fully phased sequences straight from raw reads
Proprietary software - TransMatch™
All-in-one for bioinformatics and reporting
TransMatch™ is a software for HLA sample management and results reporting, delivering end-to-end analytics and reporting for laboratory. Powered by dual‑algorithms cross‑validation, automated novel allele detection, and one‑click reporting function, it achieves exceptional HLA typing accuracy with an efficient workflow.
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Handles sample management, analysis, and reporting in one system
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Fully automated calling, user-friendly
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Proprietary dual-algorithm – take out ambiguous final call
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Regular database updates
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Flagging candidates for novel allele
End-to-End Technology Transfer
Xcelom Limited and Haorui Genomics provide a comprehensive end-to-end turnkey solution for conducting HLA typing tests within your facility.
From Sample to Report:
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Laboratory setup and consultation
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End-to-end Workflow: SOPs, reagent, and training
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Analysis Software: From sample management to report generation
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Platform
PacBio Sequel IIe, Vega and Revio system
Sample Type
gDNA from blood
Test per Batch
1 - 192 tests per SMRT cell
Operation Time
~ 40.5 hours (Hand on time: ~85 minutes)
Automation
Supported
Coverage
Basic for 11 HLA loci - Class I & II: HLA-A, HLA-B, HLA-C, HLA-DRB1, HLA-DRB3/4/5, HLA-DQA1, HLA-DQB1, HLA-DPA1, and HLA-DPB1
Extended coverage: HLA-E, HLA-F, and HLA-G
Please note that all working times are approximate and may vary depending on laboratory practices, hardware, and sample number.
Further Scope Expansion - HemoSure™ Tpying Series
In addition to our AltruType™ HLA typing solution, we offer expanded HemoSure™ blood typing panels. These solutions help service laboratories to broaden their offerings using the similar workflow, same sequencing run and analysis platform.
Different Assays. Separate Reactions. One Universal Protocol.
References:
1. Weimer ET, Montgomery M, Petraroia R, Crawford J, Schmitz JL. Performance Characteristics and Validation of Next-Generation Sequencing for Human Leucocyte Antigen Typing. J Mol Diagn. 2016;18(5):668-675.